GE17-3712-06

HisTrap^™ Excel

Cytiva 17-3712-06, pack of 5 × 5 mL

• 동의어(들): affinity resins, Ni Sepharose excel column, ion affinity chromatography column, IMAC column
• UNSPSC 코드: 23151816
• NACRES: NA.56

속성

• 유통기한: Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
• 포장: pack of 5 × 5 mL
• 제조업체/상표: Cytiva 17-3712-06
• 파라미터: 5 bar (The maximum pressure the packed bed can withstand depends on the chromatography medium characteristics and sample/liquid viscosity. The value measured on the chromatography system used also depends on the tubing used to connect the column.); 5 ml/min - 20 ml/min flow rate (2O at room temperature. Maximum flow rate will be lower when using buffers or samples with high viscosity or performing purification at low temperature. Optimal flow rate during binding is sample-dependent. During column wash and elution, a flow rate of 5 ml/min is recommended.)
• 베드 크기: 16 mm × 25 mm
• 베드 용적: 5 mL
• 컬럼 I.D.: 16 mm
• 기질: highly cross-linked 6% agarose
• 평균 직경: 90 μm
• cleaning: 2-14(Cleaning-in-place: pH interval where the medium can be subjected to cleaning-in-place without significant change in function.)
• working range: 2-13
• 용량: ≥10 mg binding capacity (histidine-tagged protein/ml sedimented medium)(Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6 tagged pure protein (Mr 43 000) in EX-CELL^® 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.)

설명

일반 설명

HisTrap^™ excel columns are prepacked with Ni Sepharose^™ excel affinity media for capture and purification of histidine-tagged proteins secreted by immobilized metal ion affinity chromatography (IMAC). The media is resistant to chelating and reducing agents making the medium an excellent choice for the purification of excreted proteins.

애플리케이션

Techniques & Applications: Ready-to-use IMAC columns, prepacked with Ni Sepharose^™ excel medium resistant to chelating and reducing agents

특징 및 장점

• HiTrap^® columns prepacked with Ni Sepharose^™ excel medium, resistant to chelating and reducing agents
  • Load eukaryotic cell culture samples containing secreted histidine-tagged proteins directly with retained binding capacity
• Increase target protein yield and decrease degradation through reduced and simplified sample handling
• HisTrap^™ excel columns allow direct purification of cell-free, unclarified samples.

저장 및 안정성

Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

안전성 정보

• 픽토그램: GHS09
• 신호어: Warning
• 유해 및 위험 성명서: H226
• 예방조치 성명서: P210 - P235 - P241 - P280 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids