추천 제품
형태
suspension
Quality Level
기술
affinity chromatography: suitable
기질
Highly cross-linked 6% Beaded Agarose
용량
15 mg/mL, gel binding capacity (protein)(with an approx. 30 kDa protein)
저장 온도
2-8°C
일반 설명
HIS-Select High Flow Nickel Affinity Gel is an immobilized metal-ion affinity chromatography (IMAC) gel that is designed to specifically bind His-tag proteins (histidine containing proteins) in chromatographic systems with pressures up to 200 psi and a maximum linear flow rate of 3,000 cm/hr.
애플리케이션
Affinity gel is suitable for the purification of histidine tagged proteins (His-tag proteins) in native and denaturing conditions.
Nickel Affinity Gel for the purification of His-tag® proteins (histidine tagged proteins). HIS-Select® High Flow (HF) brings the superior selectivity of HIS-Select technology to a highly cross-linked agarose for higher flow rates and mechanical stability under pressure. HIS-Select HF is designed for production scale purification and FPLC™ applications. As with other HIS-Select products, the non-charged, hydrophilic linkage of the nickel-nitrilotriacetic acid (Ni-NTA) chelate group to the agarose ensures true one-step purification.
특징 및 장점
- High selectivity for higher purity
- Unique non-charged hydrophilic linkage reduced non-specific binding
- Binding Capacity for histidine-tagged protein (His-tag protein) is greater than 15 mg/mL
- Highly cross-linked agarose matrix allows for purification under higher pressures
- Binding under denaturing or non-denaturing conditions
- One-step purification
물리적 형태
1:1 suspension in a 30% ethanol solution
제조 메모
Ethanol must be removed prior to use. Wash affinity gel in deionized water to remove the ethanol, then equilibrate with equilibration buffer that is provided.
법적 정보
FPLC is a trademark of Cytiva
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS-Select is a registered trademark of Merck KGaA, Darmstadt, Germany
신호어
Danger
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1A Inhalation - Flam. Liq. 3 - Muta. 2 - Repr. 1B - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 - STOT SE 2
표적 기관
Eyes,Central nervous system, Respiratory Tract
WGK
WGK 3
Flash Point (°F)
88.0 °F
Flash Point (°C)
31.1 °C
개인 보호 장비
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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Ivanova, A.S. et al.
Russian journal of developmental biology, 49, 393-397 (2018)
Hesham Saeed et al.
International journal of biological macromolecules, 106, 1041-1051 (2017-08-31)
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Nature chemical biology, 12(12), 1089-1096 (2016-10-25)
Cellular signaling is often propagated by multivalent interactions. Multivalency creates avidity, allowing stable biophysical recognition. Multivalency is an attractive strategy for achieving potent binding to protein targets, as the affinity of bivalent ligands is often greater than the sum of
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RNA biology, 15(9), 1174-1180 (2018-09-04)
Ribosome biogenesis requires a variety of trans-acting factors in order to produce functional ribosomal subunits. In human cells, the complex formed by the proteins hNob1 and hPno1 is crucial to the site 3 cleavage occurring at the 3'-end of 18S
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Journal of medicinal chemistry, 63(16), 8857-8866 (2020-06-12)
DNA-encoded small molecule libraries (DELs) have enabled discovery of novel inhibitors for many distinct protein targets of therapeutic value. We demonstrate a new approach applying machine learning to DEL selection data by identifying active molecules from large libraries of commercial
관련 콘텐츠
Pull-down assays, reagents, and protocols for investigating in vitro protein-protein interactions using affinity or GST pull-down, tandem affinity purification (TAP), and co-immunoprecipitation methods.
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