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Key Documents

GE17371201

Ni Sepharose Excel, 25 ML

Cytiva 17371201, 90 μm avg. part. size

동의어(들):

Ni Sepharose Excel

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About This Item

UNSPSC 코드:
41106500
NACRES:
NA.56

제조업체/상표

Cytiva 17371201

기질

highly cross-linked 6% agarose

평균 부품 크기

90 μm

cleaning in place

2-14

working range

2-12

용량

≥10 mg binding capacity (histidine-tagged protein)

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

)Dynamic binding capacity was tested with 0.5 mg/ml (histidine)6-tagged pure protein (Mr 43 000) in EX-CELL 420 Insect serum-free medium (capacity at 10% breakthrough) or (histidine)6-tagged protein (Mr 28 000). Column volume was 1 ml and flow rate 1 ml/min. Binding capacity is sample-dependent.
Ni Sepharose excel is a his-tagged protein purification resin for minimized Ni-leakage and maximized protein recovery when samples contain stripping agents.

EDTA-resistant resin for IMAC chromatography: Ni Sepharose excel is an IMAC resin precharged with nickel ions that are very strongly bound to a chelating ligand. Nickel ions remain bound to the ligand even after 24 h incubation in 10 mM EDTA. Because of this strong binding, samples that typically cause stripping of metal ions can be loaded directly onto the resin without time-consuming pretreatment. Therefore, this his tag purification resin is especially suitable for purifying histidine-tagged (his-tagged) proteins secreted into eukaryotic cell culture supernatants.

His tag purification of large sample volumes: The flow properties of Ni Sepharose excel nickel resin make it well-suited to purification at a wide range of scales. This resin can handle loading of large sample volumes, so you can purify low concentrations of target proteins using manual or automated methods.

Convenient prepacked columns:
  • HisTrap excel column: HiTrap format for convenient use with a syringe, a peristaltic pump, or a chromatography system.
  • His MagSepharose excel: Magnetic beads for small-scale purification and screening. They are appropriate for early stages of research when many parallel experiments are required.

특징 및 장점

  • Versatile – suitable for eukaryotic samples, including insect and CHO cells, as well as bacterial samples such as E. coli.
  • Direct sample load – of eukaryotic cell culture supernatants, eliminating the need for pretreatment of samples that usually cause nickel stripping.
  • Retained binding capacity of up to 10 mg/mL resin.
  • Simplified sample handling – fewer steps needed compared with conventional workflow.
  • Flexible formats – prepacked and loose resin formats for optimized screening and preparative his-tag purification.

저장 및 안정성

Store at 4 to 30 °C (20% ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

Sepharose is a trademark of Cytiva

픽토그램

Environment

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

How to optimize purification of histidine-tagged proteins using GE Healthcare products.

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

모두 보기

프로토콜

This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from GE Healthcare.

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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