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Key Documents

GE17-0920-03

HiTrap® IMAC High Performance

Cytiva 17-0920-03, pack of 5 × 1 mL

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모든 사진(2)

About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

포장

pack of 5 × 1 mL

제조업체/상표

Cytiva 17-0920-03

파라미터

42 psi

베드 크기

7 mm × 25 mm

베드 용적

1 mL

컬럼 I.D.

7 mm

기질

highly cross-linked 6% agarose

평균 부품 크기

34 μm

cleaning

2-14(Medium uncharged with metal ions.)

working range

3-12

용량

≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)

일반 설명

HiTrap® IMAC HP is prepacked with IMAC Sepharose™ High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent high-resolution purification of polyhistidine tagged proteins.

애플리케이션

IMAC Sepharose™ HP is supplied free of metal ions. It is charged by the user with the transition metal ion of choice (e.g. Cu2+, Zn2+, Ni2+, or Co2+); these metal ions will bind to the covalently immobilized chelating ligand on the Sepharose.

특징 및 장점

  • For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
  • Conveniently charge with your metal of choice.
  • Small bead size for high-performance purification.
  • High binding capacity.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Optimizing Purification of Histidine-Tagged Proteins

This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.

Optimizing Purification of Histidine-Tagged Proteins

How to optimize purification of histidine-tagged proteins using GE Healthcare products.

Troubleshooting Guide for Affinity Chromatography of Tagged Proteins

This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.

Characteristics of Ni Sepharose®, Ni Sepharose® excel, TALON® Superflow™, and Uncharged IMAC Sepharose® Products

This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.

프로토콜

Purification of Histidine-Tagged Proteins using HiTrap IMAC HP and HiTrap IMAC FF

This page shows how to purify histidine-tagged proteins using HiTrap IMAC HP and HiTrap IMAC FF from GE Healthcare.

Desalting/Buffer Exchange and Concentration for Affinity Chromatography of Tagged Proteins

This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.

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