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Key Documents

GE17-0729-01

SP Sepharose™ Fast Flow

Cytiva 17-0729-01, pack of 300 mL

동의어(들):

SP Sepharose, Sepharose Fast Flow Column

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About This Item

UNSPSC 코드:
41106500
NACRES:
NA.56

ligand

sulphopropyl

설명

Ion Exchanger Type (value)

포장

pack of 300 mL

제조업체/상표

Cytiva 17-0729-01

기질

6% cross-linked agarose

입자 크기

45-165 μm

평균 직경

90 μm

cleaning in place

3-14

working range

4-13

용량

70 mg binding capacity(ribonuclease A/ml medium)

적합성

suitable for bioprocess medium

일반 설명

SP Sepharose™ Fast Flow is a strong cation exchanger based on the well established Sepharose™ Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

SP Sepharose™ Fast Flow is part of the Sepharose™ Fast Flow ion exchange platform, which has been the industrial standard for ion exchange chromatography during recent decades. It is composed of crosslinked 6% agarose beads, with sulphopropyl (Q) strong cation exchange groups. SP Sepharose™ Fast Flow has high chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols. Scale-up with SP Sepharose™ Fast Flow is straight forward and the medium is available in a range of pre-packed process development tools.

As member of the BioProcess media range, SP Sepharose™ Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

특징 및 장점

  • Well-proven strong cation exchanger developed for industrial downstream processes.
  • Used extensively for capture and intermediate purification of a wide range of approved biopharmaceuticals
  • The industry standard for ion exchange chromatography during recent decades
  • High chemical stability allows for well proven CIP and sanitization protocols
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol + 0.2 M Sodium Acetate)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

Sepharose is a trademark of Cytiva

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서

Capto MMC

Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.

Principles and Standard Conditions for Different Purification Techniques

This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using GE Healthcare products.

Non-volatile and Volatile Buffer Systems

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

Selection of Purification Equipment

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

모두 보기

프로토콜

Sepharose Fast Flow: Purification with Good Resolution and Easy Scale-Up

This page covers the use of Sepharose Fast Flow for purification of proteins.

Sample Preparation in Ion Exchange Chromatography

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

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