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Key Documents

GE17-0409-01

HiTrap® Chelating High Performance

Cytiva 17-0409-01, pack of 1 × 5 mL

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모든 사진(1)

About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

ligand

iminodiacetic acid

포장

pack of 1 × 5 mL

제조업체/상표

Cytiva 17-0409-01

파라미터

42 psi

베드 크기

16 mm × 25 mm

베드 용적

5 mL

컬럼 I.D.

16 mm

기질

highly cross-linked 6% agarose

평균 부품 크기

34 μm

cleaning

2-14

working range

3-13

일반 설명

HiTrap® Chelating HP is prepacked with Chelating Sepharose™ High Performance. The medium is charged with the metalion of your choice for Immobilized Metal ion Affinity Chromatography (IMAC) and subsequent purification of polyhistidine tagged proteins.

애플리케이션

HiTrap® Chelating HP is a prepacked ready to use, column for preparative affinity chromatography. The special design of the column, together with the matrix, provide fast, simple and easy separations in a convenient format. The columns can be operated with a syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System

특징 및 장점

  • For optimizing purification of histidine-tagged Proteins using different kinds of metal ions.
  • Conveniently charge with your metal of choice.
  • Iminidoacetic acid coupled to Sepharose™ High Performance.
  • Purifying Proteins with exposed histidine groups via metal ion complex formation.
  • Simple operation with a syringe, pump or chromatographic system such as ÄKTA® design or FPLC™ System.
  • Packed with Chelating Sepharose™ High Performance
  • Simple operation with a syringe, pump or chromatographic system such as ÄKTATMdesign or FPLCTM System

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Immobilized Metal Chelate Affinity Chromatography (IMAC)

This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.

프로토콜

Performing a Separation with GE Healthcare Products Based on Poly (His) Fusion Proteins

This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap Kit, HiTrap Chelating HP and Chelating Sepharose Fast Flow products from GE Healthcare.

Converting From Linear Fow (cm/hour) to Volumetric Flow Rates (mL/min) and Vice Versa

This page shows how to convert between linear flow and volumetric flow rates in affinity chromatography.

Column Packing and Preparation Protocol for Affinity Chromatography

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.