GE17-0407-03

HiTrap^® Heparin High Performance

Cytiva 17-0407-03, pack of 5 × 5 mL

• 동의어(들): Heparin HP columns
• UNSPSC 코드: 23151816
• NACRES: NA.56

속성

• ligand: porcine heparin
• 포장: pack of 5 × 5 mL
• 제조업체/상표: Cytiva 17-0407-03
• 파라미터: 42 psi
• 베드 크기: 16 mm × 25 mm
• 베드 용적: 5 mL
• 컬럼 I.D.: 16 mm
• Matrix: highly cross-linked 6% agarose
• 평균 부품 크기: 34 μm
• cleaning: 5-10
• 작동 범위: 5-10

설명

일반 설명

HiTrap^® Heparin HP columns are prepacked with Heparin Sepharose^™ High Performance for high-resolution purification of proteins with affnity for heparin such as DNA-binding proteins, coagulation factors, lipoproteins, and protein synthesis factors.

Immobilized heparin has two main modes of interaction with proteins. It can operate as an affinity ligand with affinity for a wide range of biomolecules, including coagulation factors and other plasma proteins, lipoproteins, protein synthesis factors, enzymes that act on nucleic acids, and steroid receptors. Heparin also functions as a high capacity cation exchanger, due to its anionic sulfate groups. Gradient elution with salt is most commonly used in both cases.

Heparin is coupled to the Sepharose^™ High Performance base matrix via the N-hydroxysuccinamide coupling method, to give high capacity, performance, and low leakage levels. HiTrap^® Heparin HP is delivered in 0.2 M NaCl with 20% ethanol as bacteriostatic agent. Store HiTrap^® Heparin HP columns equilibrated with 25 mL of 20% ethanol or a buffer containing a bacteriostatic agent. Equilibration is easily performed with a syringe and the supplied Luer adapter. Seal the column with the supplied stopper and domed nut. The recommended storage temperature is 4° C to 30° C.

특징 및 장점

• Heparin binds to a wide range of biomolecules via both affinity and ion exchange mechanisms.
• Immobilized heparin is used for purification of antithrombin III, coagulation factors, and other plasma proteins.
• Simple operation with a syringe, a pump or a chromatography system such as AKTA design
• Stable over pH 5 to 10 and tolerates all commonly used aqueous buffers.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

기타 정보

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

법적 정보

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

안전성 정보

• 픽토그램: GHS02
• 신호어: Warning
• 유해 및 위험 성명서: H226
• 예방조치 성명서: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids