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모든 사진(3)

주요 문서

GE17-0404-03

HiTrap® Protein G High Performance

Cytiva 17-0404-03, pack of 2 × 1 mL

동의어(들):

HiTrap Protein G, Protein G affinity column

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About This Item

UNSPSC 코드:
23151816
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

양식

prepacked column

교차

cross-linked

포장

pack of 2 × 1 mL

제조업체/상표

Cytiva 17-0404-03

저장 조건

(20% Ehtanol)
(20% Ethanol)

파라미터

42 psi

기술

liquid chromatography (LC): suitable

베드 크기

7 mm × 25 mm

베드 용적

1 mL

컬럼 I.D.

7 mm

크기

Matrix

(cross-linked agarose, spherical)

기질 활성군

Protein G

평균 부품 크기

34 μm

cleaning

2-9

작동 범위

3-9

용량

>1 mg binding capacity (lgG/column)
25 mg binding capacity(human IgG/column)

분리 기술

affinity

저장 온도

2-8°C

일반 설명

HiTrap® Protein G HP columns are prepacked with Protein G Sepharose High Performance for fast, easy separations of IgG from different species and subclasses.
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

특징 및 장점

  • High binding capacity of 25 mg IgG/mL medium and fast binding kinetics.
  • 34 μm bead size for high performance, high-resolution purifications.
  • Binds broad range of different species and subclasses of IgG.
  • Simple and proven method giving reproducible results.
  • Designed for use with syringe, peristalic pump, or chromatography system.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

법적 정보

HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva

픽토그램

Flame

신호어

Warning

유해 및 위험 성명서

Storage Class Code

3 - Flammable liquids


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

Nimitha R Mathew et al.
Cell reports, 35(12), 109286-109286 (2021-06-24)
B cell responses are critical for antiviral immunity. However, a comprehensive picture of antigen-specific B cell differentiation, clonal proliferation, and dynamics in different organs after infection is lacking. Here, by combining single-cell RNA and B cell receptor (BCR) sequencing of

문서

This page describes automated purification of antibodies using ÄKTA chromatography systems.

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

모두 보기

프로토콜

This page shows how to perform a two-step purification of mouse monoclonal IgG1 using HiTrap Protein G HP from GE Healthcare for the capture step.

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

This page shows how to prepare samples for purification with affinity chromatography.

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