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Key Documents

GE17-5087-01

Sigma-Aldrich

HiPrep™ 26/10 Desalting

Cytiva 17-5087-01, column L × I.D. 10 cm × 26 mm, 17-132 μm particle size (dry)

동의어(들):

Desalting Column, HiPrep Desalting Column

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About This Item

UNSPSC 코드:
41115700
NACRES:
SB.52

material

polypropylene (PP)

분자량

1000-5000 Da (globular proteins)

포장

pkg of 1 ea

제조업체/상표

Cytiva 17-5087-01

저장 조건

dry media

파라미터

<15 mL sample volume
<31 mL/min flow rate
0.15 Mpa (22 psi)

베드 용적

53 mL

컬럼 길이 × I.D.

10 cm × 26 mm

기질

cross-linked dextran
polymeric

기질 활성군

dextran-epichlorohydrin phase

입자 크기

17-132 μm (dry)

cleaning

2-13

working range

2-13

분리 기술

size exclusion (SEC)

관련 카테고리

일반 설명

HiPrep™ 26/10 Desalting is a convenient, easy-to-use column prepacked 53 mL column with Sephadex® G-25 Fine for desalting and buffer exchange.

특징 및 장점

  • Provide reliable and reproducible desalting and buffer exchange with sample sizes of up to 15 mL per column.
  • Replaces dialysis for desalting and therefore saves time and reduces labor.
  • Compatible with a laboratory pump or chromatography system such as AKTA design.
  • Two or four columns can be used in series for sample sizes of 30 to 60 mL.

적합성

All commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile
for buffer exchange prior to or after different chromatographic steps.

저장 및 안정성

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

분석 메모

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

HiPrep is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva

픽토그램

Flame
GHS02

신호어

Warning

유해 및 위험 성명서

H226

Storage Class Code

3 - Flammable liquids

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

문서

Automated Purification of Antibodies Using ÄKTA Chromatography Systems

This page describes automated purification of antibodies using ÄKTA chromatography systems.

General Considerations for Purification of GST-tagged Protein

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

Performing a purification and on-column refolding of an insoluble histidine-tagged protein

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

IgG Antibody Purification with HiTrap® Protein G HP Columns

This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from GE Healthcare.

모두 보기

프로토콜

Affinity Chromatography in a Purification Strategy (CIPP)

AC separates proteins on the basis of a reversible interaction between a protein (or group of proteins) and a specific ligand coupled to a chromatography matrix. With such high selectivity and hence high resolution for the protein(s) of interest, purification levels in the order of several thousand-fold with high recovery of active material are achievable.

General Instructions for Affinity Purification Using HiTrap® Columns

This page shows general instructions for affinity purification using HiTrap columns from GE Healthcare.

Tag Removal by Enzymatic Cleavage

This page how to remove histidine tags by enzymatic cleavage using GE Healthcare products.

Conditioning Purified Membrane Proteins

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

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