G9295

Monoclonal Anti-GAPDH−Peroxidase antibody produced in mouse

clone GAPDH-71.1, purified immunoglobulin, buffered aqueous solution

• 동의어(들): Anti-G3PD, Anti-G3PDH, Anti-Glyceraldehyde-3-phosphate Dehydrogenase
• MDL number: MFCD09265360
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: peroxidase conjugate
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: GAPDH-71.1, monoclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~37 kDa
• 종 반응성: rabbit, canine, rat, hamster, monkey, mouse, turkey, bovine, mink, human, chicken
• 반응하면 안 됨: prokaryotes
• 기술: western blot: 1:35,000-1:70,000 using A431 total cell extract
• 동형: IgM
• UniProt 수납 번호: P04406
• 응용 분야: research pathology
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... GAPDH(2597) ; mouse ... Gapdh(14433) ; rat ... Gapdh(24383)

설명

일반 설명

Monoclonal Anti-GAPDH (mouse IgM isotype) is derived from the hybridoma GAPDH-71.1 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with rabbit GAPDH.

The gene glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is mapped to human chromosome 12p13. The protein localizes in the cytoplasm but can be translocated to the nucleus depending on cellular conditions.

특이성

The antibody does not crossreact with non-vertebrate and prokaryote GAPDH.

면역원

rabbit GAPDH.

애플리케이션

Monoclonal Anti-GAPDH-Peroxidase antibody produced in mouse has been used in

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• immunocytochemistry

생화학적/생리학적 작용

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a tetramer containing identical chains. It catalyzes the reversible oxidative phosphorylation of glyceraldehyde-phosphate, which is a critical energy-yielding step in carbohydrate metabolism. It binds to several proteins including actin, tubulin, amyloid precursor, polyglutamine peptides, DRPLA (dentatorubral-pallidoluysian atrophy), and huntingtin. Protein kinase Cι/λbinds and phosphorylates GAPDH. Phosphorylated GAPDH associates with cytoskeletal elements and controls microtubule dynamics in the early secretory pathway. Poly(ADP-ribose) polymerase-1 (PARP1) interacts with GAPDH and thereby mediates brain damage in the presence of oxidative/nitrosative stress. GAPDH forms a part of OCA-S, the multicomponent OCT1 (octamer-motif-binding factor) coactivator complex, which is involved in the S phase-dependent histone H2B transcription. This association is responsible for linking H2B transcriptional machinery to cell cycle regulation and to the cellular metabolic state. GAPDH is also a component of the functional GAIT (interferon-γ-activated inhibitor of translation) mRNP (messenger ribonucleoprotein). GAPDH expression is dysregulated during melanoma progression.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H317,H334
• 예방조치 성명서: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)