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Merck
모든 사진(1)

주요 문서

G8511

Supelco

β-Galactosidase from Escherichia coli

For use as a marker in SDS-PAGE

동의어(들):

β-D-Galactoside galactohydrolase, Lactase

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352200
NACRES:
NA.25

분자량

~116 kDa

포장

vial of 0.5 mg

저장 온도

2-8°C

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애플리케이션

β-Galactosidase from Escherichia coli has been used as an internal standard in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). It has been used in the protein ladder mix for optimization of microfluidic western blotting assay.

생화학적/생리학적 작용

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

물리적 특성

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

단위 정의

One unit will hydrolyze 1.0 μmole of o-nitrophenyl β-D-galactoside to o-nitrophenol and D-galactose per min at pH 7.3 at 37 °C.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

Microfluidic Western blotting of low-molecular-mass proteins.
Gerver RE and Herr AE
Analytical Chemistry, 86, 10625-10632 (2014)
Quantitative determination of titin and nebulin in poultry meat by SDS-PAGE with an internal standard.
Tomaszewska-Gras J, et al.
Meat Science, 62, 61-66 (2002)
Screening of high-risk Gaucher disease patients using dried blood spots techniques.
Filippo Vairo et al.
Gene, 523(1), 114-115 (2013-04-10)
Natalya Chernichenko et al.
JAMA otolaryngology-- head & neck surgery, 139(2), 173-182 (2013-02-23)
To examine the therapeutic effects of an attenuated, replication-competent vaccinia virus (GLV-1h68) against a panel of 5 human salivary gland carcinoma cell lines. The susceptibility of 5 salivary gland carcinoma cell lines to infection and oncolysis by GLV-1h68 was assessed
Scott W McPherson et al.
Journal of immunology (Baltimore, Md. : 1950), 190(10), 4971-4981 (2013-04-16)
Extrathymically derived regulatory T cells (iTregs) protect against autoimmunity to tissue-specific Ags. However, whether Ag-specific iTreg generation and function is limited to secondary lymphoid tissue or whether it can occur within the tissue-specific local environment of the cognate Ag remains

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