추천 제품
생물학적 소스
Escherichia coli
Quality Level
형태
suspension
분자량
540 kDa
포장
pkg of 1,500 U
제조업체/상표
Roche
농도
5.00 mg/mL
기술
activity assay: suitable
최적 pH
7
적합성
suitable for UV spectrophotometry and general use
응용 분야
life science and biopharma
배송 상태
wet ice
저장 온도
2-8°C
일반 설명
β-Galactosidase or β-D-Galactoside galactohydrolase is basically a tetramer made up of identical four polypeptide chains, each constituting 1023 amino acids. It is very specific for D-galactose and requires K+ or Na+ and Mg2+ to be fully active. β-Galactosidase enzyme with an oxygen glycosidic bond catalyzes reactions with β-d-galactopyranosides.
At 37 °C with 2-nitrophenyl-β-D-galactoside as the substrate, approximately 30 U/mg at 25 °C with lactose as the substrate; standardized with BSA.
특이성
Cleaves terminal galactose residues that are β1,4-linked to a monosaccharide, oligosaccharide, or glycopeptide.
애플리케이션
β-galactosidase has been used in enzyme-linked immunosorbent assay (ELISA). Use β-Galactosidase to produce a calibration curve in enzymatic assays.
품질
Contaminants: <0.01% GIDH, GPT, LDH, MDH, and oxaloacetate decarboxylase, each
물리적 형태
Suspension, in 3.2 M ammonium sulfate solution, pH approximately 6, crystalline
제조 메모
The ammonium sulfate preparation is stable at 2 to 8 °C until the expiration date printed on the label. Use directly for most applications, e.g., quantitation of lactose.
In the absence of ammonium sulfate, solutions of β-galactosidase should be stabilized with Mg2+ (89 mM) and a thiol reagent (1mM β-mercaptoethanol, 1 mM dithiothreitol) [Beutler, 1984]. The thiol slows the formation of enzyme dimers resulting from intramolecular disulfide bridges.
Activator: K (50 mM) is required for activation (lactose hydrolysis).
Na (50 mM) is also an activator, particularly for hydrolysis of 2-nitrophenyl-β-D-galactopyranoside.
In the absence of ammonium sulfate, solutions of β-galactosidase should be stabilized with Mg2+ (89 mM) and a thiol reagent (1mM β-mercaptoethanol, 1 mM dithiothreitol) [Beutler, 1984]. The thiol slows the formation of enzyme dimers resulting from intramolecular disulfide bridges.
Activator: K (50 mM) is required for activation (lactose hydrolysis).
Na (50 mM) is also an activator, particularly for hydrolysis of 2-nitrophenyl-β-D-galactopyranoside.
기타 정보
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
No data available
Flash Point (°C)
No data available
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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