A6805

β-N-Acetylglucosaminidase from Streptococcus pneumoniae

recombinant, expressed in E. coli, buffered aqueous solution

• 동의어(들): β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase, β-N-Acetylhexosaminidase
• CAS Number: 9012-33-3
• 효소 위원회 번호: 3.2.1.52 (BRENDA, IUBMB)
• EC Number: 232-730-2
• MDL number: MFCD00081402
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 양식: buffered aqueous solution
• 특이 활성도: ≥80 units/mg protein
• 포장: vial of ≥1.0 unit
• 외래 활성: β-galactosidase, α-mannosidase, α-fucosidase, neuraminidase, and proteases, none detected (Enzyme is expressed in glycosidase-free host.)
• 배송 상태: wet ice
• 저장 온도: 2-8°C
• 유전자 정보: Streptococcus pneumoniae R6 ... lytB(934406)

설명

애플리케이션

β-N-Acetylglucosaminidase from Streptococcus pneumoniae has been used in the removal of O-linked sugars from inositol 1,4,5-trisphosphate (InsP3) receptor type I. It has also been used in coating PVDF strips for amelogenin trityrosyl motif peptide (ATMP) and keratin 5 binding studies.

생화학적/생리학적 작용

β-N-Acetylglucosaminidase from Streptococcus pneumoniae is an extracellular glycosidase and has broad specificity over the β1-3 and β1-6 linkages present in mucins. It belongs to the glycoside hydrolase family 85 (GH85). Also called endo-β-N-acetylglucosaminidase, it cleaves at the asparagine residue of the di-N-acetylchitobiose structure in oligosaccharides and acts on fucosylated N-glycan core. Its deglycosylation functionality is exploited in studying functions of glycoproteins.

Specific for GlcNAc; not significantly active with GalNAc

This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.

단위 정의

One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at the pH 5 at 37 °C.

물리적 형태

Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable