N9376

4-Nitrophenyl N-acetyl-β-D-glucosaminide

chromogenic, ≥99% (TLC), powder

• 동의어(들): 4-Nitrophenyl 2-acetamido-2-deoxy-β-D-glucopyranoside
• 실험식(Hill 표기법): C₁₄H₁₈N₂O₈
• CAS Number: 3459-18-5
• Molecular Weight: 342.30
• Beilstein: 96193
• EC Number: 222-398-7
• MDL number: MFCD00063696
• UNSPSC 코드: 12352204
• PubChem Substance ID: 24897896
• NACRES: NA.32

속성

• product name: 4-Nitrophenyl N-acetyl-β-D-glucosaminide, ≥99% (TLC)
• 분석: ≥99% (TLC)
• 형태: powder
• 불순물: ≤0.05% Free p-nitrophenol
• solubility: water: 5 mg/mL, clear, colorless to very faintly yellow
• 저장 온도: −20°C
• SMILES string: CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc(cc2)[N+]([O-])=O
• InChI: 1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/m1/s1
• InChI key: OMRLTNCLYHKQCK-DHGKCCLASA-N

설명

애플리케이션

N-acetyl-β-D-glucosaminidase (NAG) has been used:

• as a substrate to assay N-acetyl-β-D-glucosaminidase (NAG)
• as a substrate to study chitinase ChiB1 activity
• to assess degranulation using rat basophilic leukemia (RBL)-2H3 cells

생화학적/생리학적 작용

4-Nitrophenyl N-acetyl-β-D-glucosaminide (NP-GlcNAc) is a widely used hexosaminidase substrate. Enzymatic action cleaves the glycosidic bond and forms 4-nitrophenolate (pNP) which is measured at 405 nm. NP-GlcNAc can also be used in combination with diethylaminoethyl-a-cyclodextrin (DEn-CD) for a rapid and accurate rate assay method for b-N-acetylglucosaminidase. The cyclodextrin derivative is used as an additive to ionize 4-nitrophenol to yellow-colored 4-nitrophenoxide at a pH near 5, the pH optimum for the enzyme.

기질

Chromogenic substrate for N-acetyl-glucosaminidase.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)