10354074001

Citrate Lyase (CL)

from Klebsiella pneumoniae (Aerobacter aerogenes)

• 동의어(들): Citrate Lyase (CL)
• 효소 위원회 번호: 4.1.3.6 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204

속성

• 생물학적 소스: bacterial (Klebsiella pneumoniae)
• Quality Level: 100
• 양식: lyophilized
• 특이 활성도: ≥0.25 U/mg (At 25 °C with citrate as the substrate.)
• 포장: pkg of 120 U
• 제조업체/상표: Roche
• 기술: activity assay: suitable
• 색상: light cream
• 최적 pH: 8.0-9.0
• solubility: water: soluble
• NCBI 수납 번호: WP_323133469
• UniProt 수납 번호: P45413
• 응용 분야: life science and biopharma
• 외래 활성: ICDH <0.05% (NAD as coenzyme); NADH oxidase <0.05%
• 저장 온도: 2-8°C

설명

일반 설명

Citrate lyase is considered as a substrate- induced enzyme which helps in catalyzing reversible aldol cleavage of citrate to oxaloacetate and acetate. The enzyme is adaptive to anaerobic dissimilation of citrate.

애플리케이션

It was used in enzymatic assay to measure citrate concentration using microtiter plates.

품질

Contaminants: <0.05% ICDH (NAD specific) and “NADH oxidase”, each

물리적 형태

Lyophilizate, stabilized with BSA, sucrose, MgSO₄ and EDTA. Note: A solution in double-distilled water has a pH-value of approximately 7.0. The enzyme can be relyophilized.

제조 메모

Activator: Mg²⁺ (K_m = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn²⁺, Fe²⁺, Zn²⁺)

기타 정보

For life science research only. Not for use in diagnostic procedures.

안전성 정보

• WGK: WGK 1
• Flash Point (°F): does not flash
• Flash Point (°C): does not flash