G1270

L-Glutamine Synthetase from Escherichia coli

lyophilized powder, 400-2,000 units/mg protein

• 동의어(들): L-Glutamate:ammonia ligase (ADP-forming)
• CAS Number: 9023-70-5
• 효소 위원회 번호: 6.3.1.2 (BRENDA, IUBMB)
• MDL number: MFCD00131463
• UNSPSC 코드: 12352204
• NACRES: NA.26

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: 400-2,000 units/mg protein
• 정제법: affinity chromatography
• 포함: dithioerythritol as preservative
• 구성: Protein, ~5% Lowry
• solubility: H₂O: soluble 0.95-1.05 mg/mL, clear to hazy
• UniProt 수납 번호: P0A9C5
• 외래 활성: ATPase <0.2%
• 저장 온도: −20°C
• 유전자 정보: Escherichia coli K12 ... glnA(948370)

설명

일반 설명

L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.

애플리케이션

L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.

L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.

생화학적/생리학적 작용

L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.

Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.

Degradative enzyme for glutamic acid

단위 정의

One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.

물리적 형태

Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)