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Merck
모든 사진(1)

Key Documents

F3290

Millipore

FLAG® Peptide

≥85% (HPLC), lyophilized powder

동의어(들):

Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, ddddk peptide, dykddddk peptide

로그인조직 및 계약 가격 보기


About This Item

실험식(Hill 표기법):
C41H60N10O20
Molecular Weight:
1012.97
MDL number:
UNSPSC 코드:
12352202
PubChem Substance ID:
NACRES:
NA.32

product name

FLAG® Peptide, lyophilized powder

분석

≥85% (HPLC)

Quality Level

형태

lyophilized powder

분자량

1012.97 Da

배송 상태

wet ice

저장 온도

2-8°C

SMILES string

NCCCC[C@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H](N)CC(O)=O)C(O)=O

InChI

1S/C41H60N10O20/c42-11-3-1-5-22(45-36(65)24(13-19-7-9-20(52)10-8-19)47-34(63)21(44)14-29(53)54)35(64)48-26(16-31(57)58)38(67)50-28(18-33(61)62)40(69)51-27(17-32(59)60)39(68)49-25(15-30(55)56)37(66)46-23(41(70)71)6-2-4-12-43/h7-10,21-28,52H,1-6,11-18,42-44H2,(H,45,65)(H,46,66)(H,47,63)(H,48,64)(H,49,68)(H,50,67)(H,51,69)(H,53,54)(H,55,56)(H,57,58)(H,59,60)(H,61,62)(H,70,71)/t21-,22-,23-,24-,25-,26-,27-,28-/m0/s1

InChI key

XZWYTXMRWQJBGX-VXBMVYAYSA-N

일반 설명

The FLAG peptide is used for the competitive elution of amino-terminal, Met-amino-terminal or carboxy-terminal FLAG fusion proteins from the Anti-FLAG M1 or Anti-FLAG M2 antibody (in solution or bound to an agarose gel).

애플리케이션

Gently elutes FLAG fusion proteins from ANTI-FLAG® M1 and M2 affinity resins. Usual working concentration is 100 μg/ml. Will not elute 3X FLAG fusion proteins. For this application, use 3X FLAG Peptide, F4799.

Learn more product details in our FLAG® application portal.

제조 메모

For stock solution, dissolve in TBS (10mM Tris HCL, 150 mM NaCl, pH7.4) to a final concentration of 5 mg/mL.

법적 정보

ANTI-FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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문서 라이브러리 방문

Omar H Vandal et al.
Nature medicine, 14(8), 849-854 (2008-07-22)
Acidification of the phagosome is considered to be a major mechanism used by macrophages against bacteria, including Mycobacterium tuberculosis (Mtb). Mtb blocks phagosome acidification, but interferon-gamma (IFN-gamma) restores acidification and confers antimycobacterial activity. Nonetheless, it remains unclear whether acid kills
Barbara G Mellone et al.
PLoS genetics, 7(5), e1002068-e1002068 (2011-05-19)
Semi-conservative segregation of nucleosomes to sister chromatids during DNA replication creates gaps that must be filled by new nucleosome assembly. We analyzed the cell-cycle timing of centromeric chromatin assembly in Drosophila, which contains the H3 variant CID (CENP-A in humans)
Zheng Xu et al.
Molecular cell, 35(4), 426-441 (2009-09-01)
Accurate chromosome segregation during mitosis and meiosis depends on shugoshin proteins that prevent precocious dissociation of cohesin from centromeres. Shugoshins associate with PP2A, which is thought to dephosphorylate cohesin and thereby prevent cleavage by separase during meiosis I. A crystal
Tong Zhou et al.
Nucleic acids research, 33(1), 289-297 (2005-01-14)
Tyrosyl-DNA phosphodiesterase (TDP1) is a DNA repair enzyme that removes peptide fragments linked through tyrosine to the 3' end of DNA, and can also remove 3'-phosphoglycolates (PGs) formed by free radical-mediated DNA cleavage. To assess whether TDP1 is primarily responsible
Kenjiro Hanaoka et al.
Magnetic resonance imaging, 26(5), 608-617 (2008-02-01)
Simple low molecular weight (MW) chelates of Gd(3+) such as those currently used in clinical MRI are considered too insensitive for most molecular imaging applications. Here, we evaluated the detection limit (DL) of a molecularly targeted low MW Gd(3+)-based T(1)

관련 콘텐츠

Protein purification techniques, reagents, and protocols for purifying recombinant proteins using methods including, ion-exchange, size-exclusion, and protein affinity chromatography.

Protein expression technologies for expressing recombinant proteins in E. coli, insect, yeast, and mammalian expression systems for fundamental research and the support of therapeutics and vaccine production.

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