E6154

Anti-phospho-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution

• 동의어(들): Anti-phospho-EGFR (pTyr¹⁰⁶⁸)
• MDL number: MFCD03845325
• UNSPSC 코드: 51111800
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous glycerol solution
• 분자량: antigen 170 kDa
• 종 반응성: human, rat
• 기술: western blot: suitable
• UniProt 수납 번호: P00533
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... EGFR(1956)

설명

일반 설명

The EGFR family includes four receptor tyrosine kinases, EGF R (ErbB1), ErbB2 (neu), ErbB3, and ErbB4. EGF R is present on many cell types of epithelial and mesenchymal lineages.

The gene EGFR (Epidermal Growth Factor Receptor) is mapped to human chromosome 7p11.2.

면역원

synthetic phosphopeptide derived from the region of EGFR that contains tyrosine 1068.

애플리케이션

Anti-phospho-EGFR [pTyr¹⁰⁶⁸] antibody may be used at a working dilution of 1:1000 for immunoblotting using NIH3T3 cells expressing human EGF R, stimulated with EGF. The antibody has been in detection of EGF R [pTyr¹⁰⁶⁸] in human lymphocytes.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

생화학적/생리학적 작용

The members of epidermal growth factor receptor (EGF R) or the ErbB receptor family have been identified as useful biomarkers and targets for cancer therapy. EGF R binds EGF and induces tyrosine phosphorylation leading to proliferation of cells. EGF R is capable of binding transforming growth factor-a and heparin-binding EGF in addition to EGF. There are numerous effector molecule activated by EGF R that result in a variety of biological processes such as morphogenesis, cell motility, apoptosis, differentiation and organ repair and maintenance. Deregulation of EGF R signaling is implicated in progression of a wide variety of tumors, invasion and metastasis. Phosphorylation of Tyr1068 occurs by autophosphorylation of EGF R and is required for the binding of Grb2 and activation of ERK1/2 pathway Anti-phospho-EGFR [pTyr1068] specifically recognizes human and rat EGFR phosphorylated at tyrosine 1068.

물리적 형태

Solution in Dulbecco′s phosphate buffered saline, with 50% glycerol, 1 mg/ml BSA and 0.05% sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)