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Merck
모든 사진(1)

주요 문서

DIF001

Sigma-Aldrich

3T3-L1 DIFFERENTIATION KIT

Sufficient for 100 mL differentiation medium

동의어(들):

3T3-L1 Differentiation Kit

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About This Item

UNSPSC 코드:
12352207
NACRES:
NA.75

양식

solution

사용

100 mL differentiation medium

기술

cell culture | mammalian: suitable

배송 상태

wet ice

저장 온도

−20°C

일반 설명

3T3-L1 cells are derived from mouse 3T3 cells and provide a widely-used fundamental model in the study of adipose physiology and metabolic diseases. They exhibit a fibroblast-like morphology before differentiation but become rounded and accumulate lipid droplets several days after the initiation of differentiation. The accumulated lipid droplets can be visualized by light microscopy. The in vitro differentiated 3T3-L1 adipocytes result in characteristics similar to tissue-derived adipocytes and have been commonly used to study adipogenesis, lipolysis, and metabolic dysfunctions. The 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes.

애플리케이션

3T3-L1 DIFFERENTIATION KIT has been used in:
  • Differentiation of 3T3-L1 preadipocytes to adipocytes.
  • Study of obesity, adipogenesis, lipolysis and lipid metabolism
  • Differentiation of 3T3-L1 preadipocytes to adipocytes
  • Study of obesity, adipogenesis, lipolysis and lipid metabolism.

제조 메모

Insulin (1.5 mg/ml) 0.6 ml (Catalog Number DIF001A, Green Cap)
Differentiation Cocktail, 1000x (lyophilized) 1 vl (Catalog Number DIF001B, Yellow Cap)
DMSO (anhydrous) 0.5 ml (Catalog Number DIF001C, Blue Cap)

저장 및 안정성

Store the kit at −20 °C, protected from light.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects


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문서 라이브러리 방문

Fuu-Jen Tsai et al.
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Antiretroviral (ART) drugs has previously been associated with lipodystrophic syndrome, metabolic consequences, and neuropsychiatric complications. ART drugs include three main classes of protease inhibitors (PIs), nucleoside analog reverse transcriptase inhibitors (NRTIs), and non-nucleoside reverse transcriptase inhibitors (NNRTIs). Our previous work
Jérémie Gautheron et al.
Nature communications, 7, 11869-11869 (2016-06-22)
Receptor-interacting protein kinase 3 (RIPK3) mediates necroptosis, a form of programmed cell death that promotes inflammation in various pathological conditions, suggesting that it might be a privileged pharmacological target. However, its function in glucose homeostasis and obesity has been unknown.

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