D2916

Lambda DNA Mixed Digest

for DNA electrophoresis

• UNSPSC 코드: 12352200
• NACRES: NA.25

속성

• Quality Level: 100; 200
• 양식: buffered aqueous solution
• 사용: 100 uses (0.1 mg size); 20 uses (20 μg size)
• 적합성: suitable for electrophoresis (DNA)
• 저장 온도: −20°C

설명

일반 설명

Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

애플리케이션

Lambda DNA Mixed Digest has been used for DNA electrophoresis.

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

성분

Sigma′s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

제조 메모

Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.

기타 정보

For optimal resolution, the recommended agarose gel concentration is 0.4%.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable