S7025

50 bp DNA Step Ladder

for DNA electrophoresis

• 동의어(들): DNA Ladder, Step Ladder, 50 bp, 17 Fragments, 50-3000 bp
• UNSPSC 코드: 41105335
• NACRES: NA.25

속성

• Quality Level: 100
• 형태: liquid
• 사용: 100 uses
• 적합성: suitable for electrophoresis (DNA)
• 저장 온도: −20°C

설명

일반 설명

Sigma′s 50 bp DNA Step Ladder contains 17 blunt-ended fragments consisting of 50 bp repeats from 50 to 500 bp, 100 bp repeats from 600 to 900 bp, and 1 kb repeats from 1 to 3 kb. For NA electrophoresis, 10 μl of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for Northern and Southern blotting.

애플리케이션

Suitable for size determination of PCR-generated DNA fragments with either agarose or polyacrylamide gels.

성분

Sigma′s 50 bp DNA Step Ladder is provided in a solution of 10 mM Tris-HCl (pH 7.5), 10 mM NaCl, and 1 mM EDTA.

기타 정보

Contains blunt-ended fragments of DNA, 50-500 bp by 50, 600-900 bp by 100, and 1000-3000 bp by 1000 (17 bands).

For optimal resolution, the recommended agarose gel concentration is 2.0%.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable