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Merck
모든 사진(1)

Key Documents

G7654

Sigma-Aldrich

Gel Loading Solution

for NA electrophoresis, solution

동의어(들):

DNA Gel Loading Solution, Gel Loading Buffer

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About This Item

UNSPSC 코드:
12161703
NACRES:
NA.25

Grade

for molecular biology

Quality Level

형태

solution

농도

6 ×

저장 온도

2-8°C

일반 설명

Gel loading solution is used as a tracking dye during electrophoresis. The dyes have a slight negative charge and will migrate the same direction as DNA, allowing the user to monitor the progress of molecules moving through the gel. The rate of migration varies with gel composition. Dilute 1:6 with sample before loading.

애플리케이션

Suitable for use with agarose or non-denaturing polyacrylamide gel electrophoresis (PAGE), which may be part of Northern and Southern blot hybridization procedures.

성분

Gel loading buffer contains 0.25% bromophenol blue, 0.25% xylene cyanol, and 40% sucrose.

기타 정보

Band migration can be expected as follows:
On polyacrylamide gels, xylene cyanole comigrates with approximately 450-460 bp DNA, while bromophenol blue comigrates with 15-100 bp DNA. On 0.5 – 1.4% agarose gels, xylene cyanole comigrates with 4 kb dsDNA, while bromophenol blue comigrates with 300 bp dsDNA.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

David R Macinga et al.
Antimicrobial agents and chemotherapy, 47(8), 2526-2537 (2003-07-25)
We have characterized an early series of 5,6-bridged dioxinoquinolones which behaved strikingly different from typical quinolones. The 5,6-bridged dioxinoquinolones inhibited Escherichia coli DNA gyrase supercoiling activity but, unlike typical quinolones, failed to stimulate gyrase-dependent cleavable complex formation. Analogous unsubstituted compounds
Sambrook, J., et al.
Molecular Cloning: A Laboratory Manual, 6-6 (1989)
M K Bolla et al.
Journal of lipid research, 40(12), 2340-2345 (1999-12-10)
The apoE gene exhibits two common polymorphisms that have been associated with both coronary artery disease and Alzheimer's disease. The polymorphisms create the three allelic isoforms E2, E3, and E4 which are encoded by Cys;-Cys, Cys;-Arg, and Arg;-Arg at amino
S Henry et al.
Vox sanguinis, 70(1), 21-25 (1996-01-01)
While screening Le(a+b+)Polynesian DNA samples for a candidate Se(w) allele, a point mutation (C571-->T) resulting in a new stop codon (Arg191-->stop) in the alpha(1,2)fucosyltransferase gene (FUT2) was identified. This point mutation resulted in the gaining of a new restriction enzyme
Jung-Lim Lee et al.
Journal of microbiological methods, 67(3), 456-462 (2006-12-22)
Ethidium bromide monoazide (EMA) was utilized to selectively allow conventional PCR amplification of target DNA from viable but not dead cells from a broth culture of bacterial mixed flora derived from cod fillets. The universal primers designated DG74 and RW01

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