추천 제품
Quality Level
형태
solution
분자량
32,300 kDa
포장
pkg of 1 mL
제조업체/상표
Roche
저장 조건
avoid repeated freeze/thaw cycles
농도
250 μg/mL
불순물
RNA, none detected
nuclease, none detected
색상
colorless
solubility
water: miscible
배송 상태
dry ice
저장 온도
−20°C (−15°C to −25°C)
일반 설명
The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.
애플리케이션
DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.
품질
The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
서열
Chain Length 48,502 bp
물리적 형태
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
저장 및 안정성
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.
기타 정보
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point (°F)
No data available
Flash Point (°C)
No data available
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
Bacteriophage lambda DNA: the beginning of the end.
A Becker et al.
Journal of bacteriology, 172(6), 2819-2824 (1990-06-01)
Isolation of high quality DNA and RNA from leaves of the carnivorous plant Drosera rotundifolia.
Bekesiova I, et al.
Plant Molecular Biology Reporter, 17, 269-277 (1999)
Rachel Howard-Till et al.
BioTechniques, 70(2), 120-125 (2020-12-15)
Genetic characterization of wild and cultivated plants provides valuable knowledge for conservation and agriculture. DNA sequencing technologies are improving, and costs are dropping. Yet analysis of many species is hindered because they grow in regions that lack infrastructure for advanced
Darren Yang et al.
Nature communications, 7, 11026-11026 (2016-03-18)
We present a miniature centrifuge force microscope (CFM) that repurposes a benchtop centrifuge for high-throughput single-molecule experiments with high-resolution particle tracking, a large force range, temperature control and simple push-button operation. Incorporating DNA nanoswitches to enable repeated interrogation by force
Michael R Wasserman et al.
Cell, 178(3), 600-611 (2019-07-28)
The eukaryotic replicative helicase CMG is a closed ring around double-stranded (ds)DNA at origins yet must transition to single-stranded (ss)DNA for helicase action. CMG must also handle repair intermediates, such as reversed forks that lack ssDNA. Here, using correlative single-molecule
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