D1946

DAF-FM DA

≥98% (HPLC)

• 동의어(들): 4-Amino-5-(N-methylamino)-3′,6′-bis(acetyloxy)-2′,7′-difluoro-spiro[isobenzofuran-1(3H),9′-[9H]xanthen]-3-one, Diaminofluorescein-FM diacetate
• 실험식(Hill 표기법): C₂₅H₁₈F₂N₂O₇
• CAS Number: 254109-22-3
• Molecular Weight: 496.42
• MDL number: MFCD16872050
• UNSPSC 코드: 12352200
• PubChem Substance ID: 329798717
• NACRES: NA.77

속성

• Quality Level: 100
• 분석: ≥98% (HPLC)
• 형태: film
• 저장 조건: desiccated
• 색상: colorless to yellow
• 형광: λ_ex 500 nm; λ_em 515 nm in DMSO
• 배송 상태: wet ice
• 저장 온도: −20°C
• SMILES string: CNc1ccc2c(c1N)C(=O)OC23c4cc(F)c(OC(C)=O)cc4Oc5cc(OC(C)=O)c(F)cc35
• InChI: 1S/C25H18F2N2O7/c1-10(30)33-20-8-18-13(6-15(20)26)25(12-4-5-17(29-3)23(28)22(12)24(32)36-25)14-7-16(27)21(34-11(2)31)9-19(14)35-18/h4-9,29H,28H2,1-3H3
• InChI key: BEVHTVRRVVEMEF-UHFFFAOYSA-N

설명

애플리케이션

DAF-FM DA has been used to measure the nitric oxide (NO) levels in liver tissue homogenates. It has also been used in NO staining for detection of NO from human umbilical vein endothelial cells (HUVECs).

생화학적/생리학적 작용

DAF-FM DA is a cell-permeable fluorescent probe for the detection of nitric oxide (NO). DAF-2 is the original compound and is widely used, but DAF-FM and DAF-FM DA are more sensitive to nitric oxide (NO) compared with DAF-2 and more photo-stable and less pH sensitive. DAF-FM DA permeates well into living cells and is rapidly transformed into water-soluble DAF-FM by cytosolic esterases.

특징 및 장점

This compound is featured on the Nitric Oxide Synthases page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

주의사항

Hygroscopic

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable