251500-M

DAF-2

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

• 동의어(들): DAF-2, 4,5-Diaminofluorescein
• 실험식(Hill 표기법): C₂₀H₁₄N₂O₅
• CAS Number: 205391-01-1
• Molecular Weight: 362.34
• MDL number: MFCD05865100
• UNSPSC 코드: 12171500
• NACRES: NA.25

속성

• Quality Level: 100
• 분석: ≥98% (HPLC)
• 형태: solution
• 제조업체/상표: Calbiochem^®
• 저장 조건: OK to freeze; protect from light
• 색상: pale yellow
• 형광: λ_ex ~495 nm; λ_em ~515 nm
• 저장 온도: −20°C
• InChI: 1S/C20H14N2O5/c21-15-7-11-14(8-16(15)22)20(27-19(11)25)12-3-1-9(23)5-17(12)26-18-6-10(24)2-4-13(18)20/h1-8,23-24H,21-22H2
• InChI key: LTYUPYUWXRTNFQ-UHFFFAOYSA-N

설명

일반 설명

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent triazolofluorescein compound (DAF-2T, Cat. No. 251510). Can detect NO under neutral conditions (detection limit = 5 nM).

A highly sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vivo. The relatively non-fluorescent DAF-2 reacts rapidly with NO in the presence of oxygen to yield the highly fluorescent compound triazolofluorescein (DAF-2 T). Can detect NO under neutral conditions (detection limit = 5 nM). Excitation maximum: 495 nm; emission maximum: 515 nm.

생화학적/생리학적 작용

Primary Target
Fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

경고

Toxicity: Irritant (B)

물리적 형태

A 5 mM (1 mg in 550 µl) solution in DMSO.

재구성

Following initial thaw, aliquot and freeze (-20°C).

기타 정보

Berkels, R., et al. 2000. Cell Calcium27, 281.
Kojima, H., et al. 1998. Anal. Chem. 70, 2446.
Kojima, H., et al. 1998. Chem. Pharm. Bull. 46, 373.
Nakatsubo, N., et al. 1998. FEBS Lett. 427, 263.

법적 정보

Manufactured by Daiichi Pure Chemicals Co., Ltd

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): 188.6 °F - closed cup - (Dimethylsulfoxide)
• Flash Point (°C): 87 °C - closed cup - (Dimethylsulfoxide)