D6883

2′,7′-Dichlorodihydrofluorescein diacetate

≥97% (HPLC), powder, cell-permeable non-fluorescent probe

• 동의어(들): 2′,7′-Dichlorofluorescin diacetate, DCFH-DA, 2′,7′-Dichlorodihydrofluorescein diacetate
• 실험식(Hill 표기법): C₂₄H₁₆Cl₂O₇
• CAS Number: 4091-99-0
• Molecular Weight: 487.29
• Beilstein: 4610273
• MDL number: MFCD00128955
• UNSPSC 코드: 12352200
• PubChem Substance ID: 24894058
• NACRES: NA.77

속성

• 제품명: 2′,7′-Dichlorodihydrofluorescein diacetate, ≥97%
• Quality Level: 200
• 분석: ≥97%
• 양식: powder
• 저장 온도: −20°C
• SMILES string: CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C(c2cc1Cl)c4ccccc4C(O)=O
• InChI: 1S/C24H16Cl2O7/c1-11(27)31-21-9-19-15(7-17(21)25)23(13-5-3-4-6-14(13)24(29)30)16-8-18(26)22(32-12(2)28)10-20(16)33-19/h3-10,23H,1-2H3,(H,29,30)
• InChI key: PXEZTIWVRVSYOK-UHFFFAOYSA-N

설명

일반 설명

2′,7′-Dichlorodihydrofluorescein diacetate (DCFH-DA) is an oxidation-sensitive fluorescent dye used to detect the intracellular reactive oxygen species (ROS) level. DCFH-DA is a cell-permeable non-fluorescent probe, that can be hydrolyzed by intracellular esterase to DCFH, which forms fluorescent DCF when oxidized by the intracellular ROS.

The excitation wavelength is 504 nm and emission wavelength is 529 nm in ethanol. The use of DMSO as the solvent has not been determined, however various publications suggest that it is suitable.

애플리케이션

2′,7′-Dichlorofluorescin diacetate is suitable for use in:

• sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism
• microplate assay for detecting oxidative products in phagocytic cells
• quantitative multiwell myeloid differentiation assay

Applications include sensitive and rapid quantitation of oxygen-reactive species in response to oxidative metabolism; microplate assay for detecting oxidative products in phagocytic cells, and quantitative multiwell myeloid differentiation assay.

저장 및 안정성

This product should be stored in powdered format at -20°C.

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)