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Merck
모든 사진(1)

주요 문서

CS0750

Sigma-Aldrich

Caveolea/Rafts Isolation Kit

sufficient for 20 purifications

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About This Item

UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 20 purifications

배송 상태

dry ice

저장 온도

−20°C

애플리케이션

The kit provides an easy method for obtaining fractions enriched with caveolea/rafts proteins from tissue culture cells. This method for the isolation of caveolae and rafts is based on the insolubility of these structures in ice cold non-ionic detergent Triton X-100. The kit includes all reagents for cells lysis, density gradient, and the isolation of caveolea/rafts protein enriched fraction, as well as two markers for caveolea/rafts compartments, anti-caveolin-1 and cholera toxin B subunit-peroxidase (CTB-HRP).

생화학적/생리학적 작용

Caveolae and lipid rafts are important cholesterol and sphingolipid-rich structures that represent a subcompartment or microdomain of the plasma membrane and are implicated in cellular transport and signal transduction related events.

키트 구성품 전용

제품 번호
설명

  • Lysis buffer 100 mL

  • Cholera Toxin B Subunit, Peroxidase (CTB-HRP) 50 μg

  • Protease inhibitor cocktail for use with mammalian cell and tissue extracts 1 mL

  • Anti-Caveolin-1 antibody produced in rabbit 50 μL

  • Triton X-100 2 mL

  • OptiPrep Density Gradient Medium 100 mL

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids


시험 성적서(COA)

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