Ishikawa Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 99040201, human uterus, Epithelial-like

• 동의어(들): ISHI Cells, Ishikawa Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human uterus
• 포장: tube of 5 μg 99040201-DNA-5UG; pkg of vial of cells 99040201-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial-like
• 제품: Not specified
• 수용체: Oestrogen, progesterone
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Asian endometrial adenocarcinoma

세포주 설명

The cell line Ishikawa was established from an endometrial adenocarcinoma from a 39 year old woman. The cells induced well differentiated adenocarcinoma in athymic nude mice. Estrogen and progesterone receptors were demonstrated both in cell culture and in induced tumours. Ishikawa has been reported to produce corticotropin-releasing hormone, placental alkaline phosphatase and chorionic gonadotrophin, and responded to steroid hormones. The cell line has been eradicated from mycoplasma at ECACC using BM-cyclin. Ishikawa was temporarily removed from the web site whilst an authentication issue was investigated. Following collaboration with key investigators in this field, ECACC is confident that this cell line is Ishikawa.

애플리케이션

Ishikawa Cell Line human has been used to study the effect of 1,25-Dihydroxy-Vitamin D3 (1,25(OH)2D3) on cytosolic pH regulation and glycolytic flux. It has also been used to study endometrial adenocarcinoma.

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,12
D16S539: 9
D5S818: 10,11
D7S820: 9,10
THO1: 9,10
TPOX: 8
vWA: 14,17

배양 배지

MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 5% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:5 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37ºC; cells tend to pile up.

기타 정보

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