HKT-1097

98061003, hamster kidney, Fibroblast-like

• UNSPSC 코드: 41106514

속성

• product name: HKT-1097, 98061003
• 생물학적 소스: hamster kidney
• 성장 모드: Adherent
• 핵형: Modal no. 44
• 형태학: Fibroblast-like
• 제품: Not specified
• 수용체: EGF
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Syrian Hamster kidney

세포주 설명

The cell line HKT-1097 has been established from a 14 month old male Syrian Hamster (Mesocricetus auratus) kidney tumour which has been induced by diethylstilbestrol exposure. HKT-1097 cells express epidermal growth factor (EGF) receptor, Erb-B2 and Erb-B3 as well as vimentin, desmin, glial fibrillary acidic protein (GFAP), TGF alpha, PGP 9.5 and S100 protein and neuron-specific enolase. Karyotype analysis revealed a modal number of 44 chromosomes (41% of cells) and polyploidy in approximately 2% of cells. The cells have a doubling time of about 36 hours.

DNA 프로파일

Not specified

배양 배지

DMEM + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4, i.e., seeding at 1 x 10,000 cells / cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Form a multipolar, loosely packed monolayer.

기타 정보

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