293-PERV-PK-CIRCE

97051411

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human kidney
• 성장 모드: Adherent aggregates
• 핵형: Not specified
• 형태학: Not specified
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human embryo kidney, infected with endogenous retrovirus of pigs

세포주 설명

293-PERV-PK-CIRCE has been established by infecting the human kidney 293 cell line (Sigma Catalogue number. 85120602) with retrovirus produced by the pig kidney cell line PK-15. The endogenous, C-type retrovirus particles produced by PK-15 have been shown to be capable of infection, replication and serial transfer. 293-PERV-PK-CIRCE is useful to examine the host range of the pig retrovirus to assess the risks associated with porcine xenotransplantation. The cell line has tested negative for Hog Cholera virus. Handle under ACDP Category 2 conditions.

애플리케이션

Pig retrovirus studies, infectivity assays

배양 배지

DMEM + 2mM Glutamine + 10% HI-FBS

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:6 cells can be dislodged by mechanical agitation 5% CO₂ 3

기타 정보

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