OE19 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96071721, human esophagus, Epithelial

• 동의어(들): JROECL19, JROECL19 Cells, OE-19 Cells, OEC19 Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human esophagus
• 포장: tube of 5 μg 96071721-DNA-5UG; pkg of vial of cells 96071721-1VL
• 성장 모드: Adherent
• 핵형: Aneuploid
• 형태학: Epithelial
• 제품: Not specified
• 수용체: MHC class I
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian oesophageal carcinoma

세포주 설명

The cell line OE19, also known as JROECL19, was established in 1993 from an adenocarcinoma of gastric cardia/oesophageal gastric junction of a 72 year old male patient. The tumour was identified as pathological stage III (UICC) and showed moderate differentiation. The cell line OE19 expresses HLA-A, -B and -C antigens (MHC class I) constitutively. Treatment with interferon-gamma induced the expression of ICAM-1 (CD54). Expression of HLA-DR (MHC class II) on interferon-gamma addition was only measured in a sub-population of OE19. The cells express epithelial cytokeratins and are tumourigenic in nude mice.The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

애플리케이션

OE19 cell line has been used to study the effect of microRNA-MiR-193b on autophagy and cell death on these cells. It has also been used to study microRNA-330-5p as a modulator of neoadjuvant chemoradiotherapy sensitivity.

Study of oesophageal cancer

DNA 프로파일

STR-PCR Data:
Amelogenin: X
CSF1PO: 11,13
D13S317: 9,11
D16S539: 12,13
D5S818: 11,14
D7S820: 8
THO1: 8,9
TPOX: 8
vWA: 16,17

배양 배지

RPMI 1640 + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:8, i.e., seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. These cells grow slowly in islands and when resuscitated from frozen it can take up to 7 days to reach 70% confluence.

기타 정보

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