M15

95102517, mouse kidney, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: M15, 95102517
• 생물학적 소스: mouse kidney
• 성장 모드: Adherent
• 핵형: Hyperdiploid
• 형태학: Epithelial
• 제품: WT1 (expressed gene)
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse mesonephric epithelium, polyoma virus large T transformed

세포주 설명

The WT1-expressing mesonephric cell line M15 (alias Meso15) was established from mouse mesonephros transgenically expressing the large T protein of polyoma virus under the control of the early viral enhancer. As a tumour suppresser gene with a key role in urogenital development, WT1 is implicated as predisposition gene in the pathogenesis of Wilms′ tumour (WT).

애플리케이션

Gene expression and protein studies connected to kidney development and Wilms′ tumourigenesis.

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. NB: Cell growth is impaired at lower densities.

기타 정보

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