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Merck
모든 사진(1)

주요 문서

07-405

Sigma-Aldrich

Anti-PRMT5 Antibody

Upstate®, from rabbit

동의어(들):

72 kDa ICln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog, SKB1 homolog (S. pombe), Shk1 kinase-binding protein 1 homolog, protein arginine methyltransferase 5, skb1 (S. pombe) homolog

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41

생물학적 소스

rabbit

Quality Level

항체 형태

purified immunoglobulin

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성

mouse, human

제조업체/상표

Upstate®

기술

immunoprecipitation (IP): suitable
western blot: suitable

동형

IgG

NCBI 수납 번호

UniProt 수납 번호

배송 상태

wet ice

타겟 번역 후 변형

unmodified

유전자 정보

human ... PRMT5(10419)

일반 설명

Arginine methylation of proteins is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing. PRMTs are divided into two types defined by their activity. Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues. Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues. Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins. PRMT5 (also known as Skb1Hs/JBP1) exists as homooligomeric complexes, which includes a dimer and tetramer. Homooligomerization of PRMT5 has an important role in its ability to methylate the MBP protein.
PRMT5 (aka JBP1 Janus Binding Protein1) is a member of a family of enzymes that deposit methyl groups on arginine residues of proteins, including both histone and non-histone substrates.

특이성

PRMT5, Mr ~70 kDa

면역원

KLH-conjugated, synthetic peptide corresponding to amino acids 60-79 (KNRPGPQTRSDLLLSGRDWN) of human protein arginine methyltransferase 5, SKB1 homolog. The immunizing sequence is identical in mouse.

애플리케이션

Anti-PRMT5 Antibody is a Rabbit Polyclonal Antibody for detection of PRMT5 also known as 72 kDa ICln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog & has been validated in IP & WB.
Immunoprecipitation:
An independent laboratory has reported that this antibody immunoprecipitates active PRMT5 from HeLa cell lysate.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

품질

Routinely evaluated by Western Blot in RIPA lysates from 3T3/A31, NIH 3T3,HeLa, WI-38, and Jurkat cells.

Western Blot Analysis:
0.5-1 μg/mL of this lot detected PRMT5 in RIPA lysates from 3T3/A31, NIH 3T3, HeLa, WI-38, and Jurkat cells.

표적 설명

~70 kDa

결합

Replaces: MABE551

물리적 형태

Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 70% storage buffer (0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide) and 30% glycerol. Store at -20°C.

저장 및 안정성

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

분석 메모

Control
Jurkat cell lysate, HeLa cell lysate.

Included Positive Antigen Control:
Catalog # 12-303, Jurkat lysate. Add 2.5 μL of 2- mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

기타 정보

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

법적 정보

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


시험 성적서(COA)

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문서 라이브러리 방문

Protein arginine methyltransferase Prmt5-Mep50 methylates histones H2A and H4 and the histone chaperone nucleoplasmin in Xenopus laevis eggs.
Wilczek, C; Chitta, R; Woo, E; Shabanowitz, J; Chait, BT; Hunt, DF; Shechter, D
The Journal of Biological Chemistry null
PRMT5 is required for human embryonic stem cell proliferation but not pluripotency.
Gkountela, S; Li, Z; Chin, CJ; Lee, SA; Clark, AT
Stem Cell Reviews null
Alternative splicing regulates Prdm1/Blimp-1 DNA binding activities and corepressor interactions.
Morgan, MA; Mould, AW; Li, L; Robertson, EJ; Bikoff, EK
Molecular and cellular biology null
Sarah Leonard et al.
Pathogens (Basel, Switzerland), 1(1), 52-64 (2012-01-01)
Although there is increasing evidence that aberrant expression of those enzymes which control protein arginine methylation contribute to carcinogenesis, their de-regulation by oncogenic viruses in primary cells has yet to be reported. We first show that the protein arginine methyltransferases
The histone-binding protein COPR5 is required for nuclear functions of the protein arginine methyltransferase PRMT5.
Lacroix, M; El Messaoudi, S; Rodier, G; Le Cam, A; Sardet, C; Fabbrizio, E
EMBO Reports null

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