CHO/dhFr-

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 94060607, hamster ovary, Epithelial

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: hamster ovary
• 포장: tube of 5 μg 94060607-DNA-5UG; pkg of vial of cells 94060607-1VL
• 성장 모드: Adherent
• 핵형: Modal no. 20
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Hamster Chinese ovary

세포주 설명

The CHO/dhFr- cell line lacks the enzyme dihydrofolate reductase (DHFR) which is necessary for purine synthesis. In the absence of exogenous purines, this enzyme is required for growth. Hence the CHO/dhFr- cell line has growth requirements for hypoxanthine (or adenine), glycine and thymidine. However, the cells will continue to grow without the addition of hypoxanthine and thymidine because the cells can obtain sufficient from the medium. α MEM minus does not contain the ribonucleosides or the deoxyribonucleosides, and will not support the growth of CHO/dhFr- or its serum free adaptation as neither contains the gene to produce DHFR. (See attached data sheet).

애플리케이션

CHO/dhFr- has been used to study the relation between local chromatin mobility and transcriptional activity. It has also been used as a transfection lineage and also as a negative control in cytofluorometry experiments.

배양 배지

IDMEM + 2mM Glutamine + 0.1mM Hypoxanthine + 0.01mM Thymidine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C

기타 정보

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