CHO-K1/SF

93061607, hamster ovary, Epithelial

• UNSPSC 코드: 41106514

속성

• product name: CHO-K1/SF, 93061607
• 생물학적 소스: hamster ovary
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Hamster Chinese ovary (MEM adapted)

세포주 설명

This MEM adapted sub-line of CHO-KI is used in Pertussis Toxin research and for quality control of batch production.

애플리케이션

CHO-K1/SF has been used to sequence the microRNA transcriptome and to identify, annotate and profile microRNAs as targets for cellular engineering. It has also been used to determine the pertussis toxin (PT) content of purified Bordetella pertussis fimbriae.

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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