COLO 853

93052606, human lymph, Epithelial

• UNSPSC 코드: 41106514
• 생물학적 소스: human lymph
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Melanin
• 수용체: Not specified
• 관련 질환(들): cancer

속성

• 제품명: COLO 853, 93052606
• 생물학적 소스: human lymph
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Melanin
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human malignant melanoma

세포주 설명

Derived from the right axilla lymph node of a 43 year old male with melanoma.COLO 839 (Sigma Catalogue number 93051119), COLO 858 (Sigma Catalogue number 93052613) and COLO 857 (Sigma Catalogue number 93051120) were all derived from the same 43 year-old male patient with malignant melanoma. This has been confirmed by STR profiling; COLO 853 also has an identical STR profile to the above cell lines though this cell line is not mentioned in the literature.

애플리케이션

Tumourigenicity studies

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11,12
D16S539: 13
D5S818: 11
D7S820: 8,10
THO1: 10
TPOX: 8,11
vWA: 17,19

배양 배지

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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