293 N3S

92052131, human kidney, Not specified

• UNSPSC 코드: 41106514

속성

• 제품명: 293 N3S, 92052131
• 생물학적 소스: human kidney
• 포장: tube of 5 μg 92052131-DNA-5UG; pkg of vial of cells 92052131-1VL
• 성장 모드: Suspension
• 핵형: Not specified
• 형태학: Not specified
• 제품: Adenovirus 5 early region (E1) antigens
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human embryo kidney

세포주 설명

Adapted for growth in suspension by passaging 293 cells in nude mice. The cells express adenovirus 5 early region 1 (E1) antigens and are permissive for adenovirus 5 host range mutants defective in E1 functions.

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 12,14
D16S539: 9
D5S818: 9
D7S820: 11,12
THO1: 7,9.3
TPOX: 11
vWA: 16,19

배양 배지

Joklik′s MEM + 2mM Glutamine + 10% Horse Serum.

계대배양 정규 작업

Maintain cultures between 5 x 10,000 to 5 x 100,000 cells/ml in a Techne stirrer; 5% CO₂; 37°C. Cell doubling time is 30 hours.

기타 정보

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