M-MSV-BALB/3T3

90030802, mouse embryo, Fibroblast

• UNSPSC 코드: 41106514
• 생물학적 소스: mouse embryo
• 성장 모드: Adherent
• 핵형: Modal no. 57
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 관련 질환(들): cancer

속성

• 제품명: M-MSV-BALB/3T3, 90030802
• 생물학적 소스: mouse embryo
• 성장 모드: Adherent
• 핵형: Modal no. 57
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse BALB/3T3 embryo, Moloney sarcoma virus transformed

세포주 설명

Derived from BALB/3T3 clone A31 (Sigma Catalogue number. 86110401) by infection with M-MSV. A M-MSV non-producer, but addition of MuLV permits rescue of the MSV genome. Cells produce tumours in suitable hosts; lack contact inhibition in vitro cultures; morphologically identical to virus releasing lines.

애플리케이션

Transformation and virus studies: M-MSV, MuLV

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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