WI 38 Cell Line human

90020107, human lung (fetal), Fibroblast-like

• 동의어(들): WI38 Cells, Wistar Institute-38 Cells
• UNSPSC 코드: 41106514

속성

• product name: WI 38 Cell Line human, caucasian fibroblast-like fetal lung cell, 90020107
• 생물학적 소스: human lung (fetal)
• 성장 모드: Adherent
• 핵형: 2n = 46, diploid except at high passage number
• 형태학: Fibroblast-like
• 제품: Collagen
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian foetal lung

세포주 설명

From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37°C with periodic pH adjustments. Used in preparation of human virus vaccines.

애플리케이션

From normal female embryonic lung (expect collagen production). These cells have a very broad human virus spectra. Particularly useful for the isolation of rhinovirus. Cells from multilatered membrane when incubated for long periods with periodic pH adjustments. Used in the preparation of human virus vaccines.

Subculture: Spit at 70-80% confluency, approx. 1:3 to 1:6, seed at 2-4x10⁴ viable cells/cm². Trypsinize using 0.25% solution, with or without EDTA, 37 °C and 5% CO₂.

Virus studies (rhinoviruses), vaccine production

WI 38 human cell line has also been used to study the cytotoxicity of acrylic based resin compounds and silica nanoparticles.

생화학적/생리학적 작용

Finite lifespan. Approx. 50, +/- 10, population doublings. Normal doubling time is 24 hours.

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C. Finite lifespan, 50 plus or minus 10 population doublings expected - doubling time 24h.

분석 메모

Recommended culture medium: EMEM (EBSS) + 2 mM L-Glutamine + 1% Non-essential amino acids (NEAA) + 15% FBS

기타 정보

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