A9HT

88042804, mouse unknown/unspecified, Fibroblast

• UNSPSC 코드: 41106514

속성

• 제품명: A9HT, 88042804
• 생물학적 소스: mouse unknown/unspecified
• 성장 모드: Adherent
• 핵형: Hyperdiploid
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

A9 tumour induced C3H mouse

세포주 설명

This cell line was selected by passaging A9 (Sigma Catalogue number. 85011426) through syngenic C3H or semi allogenic mice over a period of three months and uptake was considerably higher than A9, thus A9 high uptake or A9HT. Karyotype is similar to A9 but with a slightly reduced total number of chromosomes, with the modal number of 53. This line lacks inosinic acid pyrophosphorylase.

애플리케이션

Tumourigenicity studies

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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