A7r5 Cell Line from rat

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 86050803, rat muscle, Fibroblast

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: rat muscle
• 설명: Rat BDIX smooth muscle embryonic aorta
• 포장: tube of 5 μg 86050803-DNA-5UG; pkg of vial of cells 86050803-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Fibroblast
• 제품: Muscle-type isoenzymes
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rat BDIX smooth muscle embryonic aorta

세포주 설명

Flat ribbon-like cells differentiating to parallel arrays of spindle shaped cells. Derived from the thoracic aorta of an embryonic BDIX rat. As cells achieve the stationary phase there is an increase in myokinase and creatine phosphokinase (CPK) activity. After cessation of cell division a muscle type CPK isoenzyme is synthesised.

애플리케이션

A7r5 Cell Line from rat has been used to study the effect of angiotensin II on vascular oxidative stress. It has been used in PLA2 inhibitory study to understand the role of PLA2 on formation of lipid droplets.

Biochemical and biophysical studies

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. After resuscitation cells may take up to 7 days before a monolayer forms and growth can be very slow in general. Cells also grow in clumps.

기타 정보

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