EL4 Cell Line murine

85023105, mouse blood, Lymphoblast

• UNSPSC 코드: 41106514

속성

• product name: EL4 Cell Line murine, 85023105, Lymphoblast from blood
• 생물학적 소스: mouse blood
• 성장 모드: Suspension
• 핵형: 2n = 39
• 형태학: Lymphoblast
• 제품: A surface antigen induced by leukaemia type G virus; H-2b and Thy-1.2 antigens; Interleukin 4 (IL-4), H-2b, Thy1.2
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse ascites lymphoma lymphoblast

세포주 설명

Established from a lymphoma induced in a C57BL/6N mouse by 9,10-dimethyl-1,2-benzanthracene. The cells have been reported to produce low levels of type G (Gross) virus antigen histone-2b (H-2b), interleukin-4 (IL-4) and Thy-1.2 antigen. The cell line is resistant to cortisol and dexamethasone and is sensitive to polyhydroxyalkanoates (PHAs).

애플리케이션

EL4 Cell Line murine has been used to induce tumor in mice and to assess the extravasation of tumor necrosis factor (TNF)-induced Evans blue dye and E1- and E3-deleted Ad vectors carrying firefly luciferase reporter gene under cytomegalovirus promoter (AdLuc). It has also been used as a constituent of bone marrow-derived macrophage (BMDM)-polarizing media to polarize BMDM and assess its tumoricidal activity.

Tumourigenicity studies

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cell growth after resuscitation is slow. Subculture once exponential growth is established.

기타 정보

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