B82

85011408, mouse adipose tissue, Fibroblast

• UNSPSC 코드: 41106514

속성

• product name: B82, 85011408
• 생물학적 소스: mouse adipose tissue
• 성장 모드: Adherent
• 핵형: 2n = 51
• 형태학: Fibroblast
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Mouse C3H/An sc areolar and adipose tissue

세포주 설명

Derivative of NCTC clone 929 (Sigma Catalogue number. 88102702) obtained by progressive selection in BUdR, has a cloning efficiency of 70-80%.

애플리케이션

Chromosome analysis and hybridisation studies

배양 배지

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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