W6/1

13082201, from Mouse x Mouse Hybridoma

• UNSPSC 코드: 41106514

속성

• 생물학적 소스: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• 골수종: P3-NS1/1-Ag4.1
• 반응성: Human ′A′ erythrocytes
• 기술: cell culture | mammalian: suitable
• 동형: IgM
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 설명

The W6/1 hybridoma cell line secretes monoclonal IgM antibodies reactive with a polymorphic determinant of human erythrocytes which correlated with blood group A.

면역원

Human tonsil membrane

애플리케이션

Human erythrocyte marker.

배양 배지

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

계대배양 정규 작업

Seed at 5 x 10⁴ cells/ml. Subculture when cells reach 5 x 10⁵ -1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. Start in 20% FBS when recovering from frozen.

기타 정보

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.