LIM2551

12070505, human colon, epithelial; forms aggregates of small rounded cells, adherent and suspended.

• 동의어(들): LIM 2551, LIM-2551
• UNSPSC 코드: 41106514

속성

• 제품명: LIM2551, 12070505
• 생물학적 소스: human colon
• 성장 모드: Semi-adherent
• 핵형: Not specified
• 형태학: Epithelial; grows as loose aggregates of small rounded cells, some adherent, others in suspension.
• 수용체: Not specified
• 관련 질환(들): cancer

설명

세포주 기원

Human derived from an adenocarcinoma of the transverse colon

세포주 설명

LIM2551 was derived from an adenocarcinoma of the transverse colon. Cell Bank Australia reference number: CBA0170.

DNA 프로파일

Not specified

배양 배지

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + FBS / FCS (F2442), Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM.

계대배양 정규 작업

Optimal split ratio 1:8-1:16 (seeding density 1 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. LIM2551 requires the growth medium to be changed 3 times each week. Feed and passage as for semi-adherent culture (collect medium with floating cells and then trypsinise the adherent population, pool both together before reseeding).

기타 정보

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Depositor and originator: Professor Tony Burgess - Ludwig Institute for Cancer Research Ltd, Melbourne, Australia