P0372

Anti-Podocin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Podocin Antibody, Podocin Antibody - Anti-Podocin antibody produced in rabbit
• MDL number: MFCD06798508
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 형태: buffered aqueous solution
• 분자량: antigen ~42 kDa (doublet)
• 종 반응성: human, rat, mouse
• 기술: indirect immunofluorescence: 10-20 μg/mL using acetone-fixed human or rat kidney frozen sections; western blot (chemiluminescent): 0.5-1 μg/mL using whole extract of rat glomeruli
• UniProt 수납 번호: Q9NP85
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... NPHS2(7827) ; mouse ... Nphs2(170484) ; rat ... Nphs2(170672)

설명

일반 설명

Podocin is a podocyte structural protein, that belongs to the band-7-stomatin family of lipid raft-associated proteins. This family contains more than 1800 conserved proteins which share ∼150 amino acid domains showing similarity to mitochondrial protein prohibitin (PHB).
Podocin is a hairpin-like integral membrane protein with intracellular N- and C- termini. Podocin is located at the insertion site of the slit membrane, an intercellular junction found in mammalian kidney.

특이성

Anti-Podocin recognizes human, mouse, and rat podocin.

면역원

Synthetic peptide corresponding to amino acid residues of human podocin with an N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in mouse and differs by one amino acid in rat.

애플리케이션

Anti-Podocin antibody produced in rabbit has been used for Western Blotting and immunohistochemistry.

생화학적/생리학적 작용

Podocin and nephrin are essential for maintaining the slit diaphragm. Studies show that mutations in this gene account for around 15% cases of nephrotic syndrome cases. Podocin interacts with cholesterol and promotes the formation of protein-lipid supercomplexes, which control ion channel complexes. The podocin gene is known to be associated with autosomal recessive SRNS (nephrotic syndrome type 2). Podocin actively participates in the ultrafiltration process occuring in kidney.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For continuous use, store at 2-8 °C for up to one month.
For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable