293T cell line human

12022001, human kidney, fetal. Small slightly rounded cells, dispersed growth

• 동의어(들): 293T cell line human, 293 T, 293tsA1609neo, 293tsA1609neo Cells, HEK-293-T Cells, HEK-293T Cells, HEK293T Cells, Human Embryonic Kidney 293T Cells
• UNSPSC 코드: 41106514

속성

• 제품명: 293T cell line human, NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA, 12022001, human kidney, fetal. Small slightly rounded cells, dispersed growth
• 생물학적 소스: human kidney (fetal)
• 포장: tube of 5 μg 12022001-DNA-5UG; pkg of vial of cells 12022001-1VL
• 성장 모드: adherent
• 형태학: small slightly rounded cells growing in a dispersed manner
• 제품: SV40 T antigen
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

일반 설명

293T cell line is a stable clone derivative of the human embryonic kidney (HEK) 293 cell line. It expresses the large T antigen of simian virus 40 (SV40).

세포주 설명

The 293T cell line was created in the laboratory of Michele Calos by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is competent for replication of vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, transfection, DNA replication, gene expression, and protein production properties. It gives high titres when used to produce many viral vectors such as oncoretroviruses and lentiviruses.

애플리케이션

293T cell line human has been used:

• for lentiviral particle production
• in the expression of eukaryotic proteins
• for small interfering RNA (siRNA) transfection
• for human immunodeficiency virus (HIV) transfection for pseudoviruses production
• as a host for the transfection of pLKO.1.-TRC-ACE2 plasmid to study the localization and role of NL63-N, a nucleocapsid protein of human coronavirus NL63, in cell cycle progression

생화학적/생리학적 작용

Due to the presence of T antigen, 293T cell line favors simian virus 40 (SV40) plasmid replication as well as high protein expression. It also possesses stem cell properties. HEK293T is used for large-scale vector production, displays increased cell growth and is efficient for transfection experiments when compared to HEK293 cells.

포장

NOTE: Both the cell line and DNA from the cell line may beavailable for this product. Please choose -1VL or VIAL for cells, or -DNA-5UGfor DNA.

DNA 프로파일

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19

배양 배지

DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2-4 x 10⁴ cells/cm².

기타 정보

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