RCC4plusVHL

3112703, human kidney, Epithelial

• 동의어(들): RCC4 VHL(+) Cells, RCC4-pVHL Cells, RCC4/VHL Cells
• UNSPSC 코드: 41106514
• 생물학적 소스: human kidney
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 관련 질환(들): cancer

속성

• 제품명: RCC4plusVHL, 03112703
• 생물학적 소스: human kidney
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): cancer
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Renal cell carcinoma cell line RCC4 stably transfected with pcDNA3-VHL

세포주 설명

Renal cell carcinoma cell line RCC4 stably transfected with pcDNA3-VHL. The plasmid pcDNA3-VHL confers neomycin resistance and encodes the von Hippel-Lindau (VHL) tumour suppressor gene product pVHL. The original renal carcinoma cell line RCC4 is VHL-deficient. The RCC4 plus vector alone cell line (Sigma catalogue no. 03112702) can serve as a negative control cell line to study the effect of pVHL expression from pcDNA3-VHL. These cells are less tumourigenic than corresponding cell lines lacking pVHL function.

애플리케이션

Study of tumour suppressor gene product function - von Hippel-Lindau tumour suppressor gene. Cell responses to hypoxia.

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 11
D5S818: 12
D7S820: 8.2
THO1: 9.3
TPOX: 11
vWA: 16

배양 배지

DMEM + 10% FBS + 2 mM Glutamine + G418 0.5mg/ml

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 to 1:8 using 0.25% Trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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