RK-13 Cell Line from rabbit

rabbit kidney, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 21715, rabbit kidney, Epithelial-like

• 동의어(들): GL RK/13 Cells, Glaxo Laboratories Rabbit Kidney 13 Cells, R13 Cells
• UNSPSC 코드: 41106514
• eCl@ss: 32190102

속성

• 생물학적 소스: rabbit kidney
• 포장: tube of 5 μg 00021715-DNA-5UG; pkg of vial of cells 00021715-1VL
• 성장 모드: Adherent
• 핵형: 2n=44, stem-line = 56 (=22%)
• 형태학: Epithelial-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Rabbit kidney

세포주 설명

RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.

애플리케이션

Virus studies

배양 배지

EMEM(HBSS) (M5775) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37ºC; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical.

기타 정보

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.