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Merck
모든 사진(2)

주요 문서

CASP3F

Sigma-Aldrich

Caspase 3 Assay Kit, Fluorimetric

동의어(들):

Caspase-3 Activity Fluorometric Kit

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About This Item

EC Number:
UNSPSC 코드:
12161503
NACRES:
NA.84

사용

sufficient for 1,000 multiwell tests
sufficient for 100 standard tests (2 ml)

Quality Level

배송 상태

dry ice

저장 온도

−20°C

유전자 정보

human ... CASP3(836)

일반 설명

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

애플리케이션

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

단위 정의

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

키트 구성품 전용

제품 번호
설명

  • Water (17-18 megaOhm) 125 mL

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS

  • A1086Ac-DEVD-AMC substrate 2.5 mgSDS

  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS

  • A98917-Amino-4-methylcoumarin standard 1 mgSDS

픽토그램

Exclamation mark

신호어

Warning

유해 및 위험 성명서

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

N M Martins et al.
Journal of applied toxicology : JAT, 28(3), 337-344 (2007-07-03)
Cisplatin is a potent and widely used chemotherapeutic agent. Nephrotoxicity induced by this drug has been well documented. However, very little information is available on cisplatin-induced hepatotoxicity and its underlying mechanism remains unclear. High doses of cisplatin have been known
Radharaman Ray et al.
Drug and chemical toxicology, 28(1), 105-116 (2005-02-22)
Sulfur mustard [SM; bis-(2-chloroethyl) sulfide], which causes skin blistering or vesication [(1991). Histo- and cytopathology of acute epithelial lesions. In: Papirmeister, B., Feister, A. J., Robinson, S. I., Ford, R. D., eds. Medical Defense Against Mustard Gas: Toxic Mechanisms and
Rebeca Vecino et al.
Scientific reports, 8(1), 1610-1610 (2018-01-27)
Brain preconditioning (PC) refers to a state of transient tolerance against a lethal insult that can be evoked by a prior mild event. It is thought that PC may induce different pathways responsible for neuroprotection, which may involve the attenuation
Ashish Kumar et al.
Scientific reports, 8(1), 2-2 (2018-01-10)
Recently, we have demonstrated that microRNA-31 (miR-31) overexpression is inherent to radiation-induced cell death in the highly radioresistant Sf9 insect cells, and regulates pro-apoptotic Bax translocation to mitochondria. In the present study, we report that at sub-lethal radiation doses for
Joana Silva et al.
BMC complementary and alternative medicine, 18(1), 58-58 (2018-02-16)
Parkinson's disease (PD) is a progressive neurodegenerative disorder of the central nervous system. Although the causes of PD pathogenesis remain incomplete, some evidences has suggested that oxidative stress is an important mediator in its pathogenesis. The aim of this study

문서

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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